DNA/RNA Sample Quantification and Purity Services

Introduction

All samples have to be accompanied by appropriate sample QC documentation (e.g. Bioanalyzer traces, Agarose gel electrophoresis images). It speeds up the projects if this QC can be carried out as early as possible in your lab. However, we are happy to run the sample QC for you, for a fee.

Sample Submission

Starting material for Illumina library construction can be double-stranded (ds) DNA from any source: genomic DNA, BACs, PCR amplicons, ChIP samples, any type of RNA turned into ds cDNA (mRNA, normalized total RNA, smRNAs), etc. Pretty much anything you can think of that ends up as, or can be turned into, dsDNA. This dsDNA is then fragmented (if it is not already, as in ChIP). The average fragment length should not exceed 600 bp (HiSeq 2500, MiSeq) or 350 bp (HiSeq 3000 &4000). Then the ends are repaired and ‘A’ tailed, adapters are ligated on, size selection is carried out, then PCR performed to generate the final library ready for sequencing.

CD Genomics has already updated its technology platforms to mainstream NGS, long-read sequencing, and microarray instruments. We continue to strive to offer the same reliable services to pharmaceutical and biotechnology companies, as well as academia and government agencies, for all your sequencing or array needs. If you are interested, please contact us directly for assistance.