NGS Service—BioRad QX200 Digital PCR System

Summary

Use the QX200 Droplet Digital PCR (ddPCR^™) system, the most precise and sensitive PCR solution, for absolute quantification of target DNA molecules.

Applications

1. Cancer biomarker studies and copy number variation
2. Measure varying degrees of cancer mutations, detect rare DNA target copies, and resolve copy number variation states with superior sensitivity and resolution. PrimePCR^™ ddPCR Assays are now available for mutation and copy number detection. These are predesigned, wet-lab validated assays.
3. Pathogen detection
4. Employ the extreme precision of the QX200 System to quantify small fold changes in target DNA or RNA molecules in pathogen detection and monitoring.
5. Next generation sequencing
6. Perform accurate quantification and qualification of NGS libraries without the use of a standard curve.
7. Gene expression analysis
8. Achieve reliable and reproducible measurements of small fold changes for low abundance of mRNA and miRNA.
9. Environmental monitoring
10. Test a wide variety of environmental samples like soil and water using the QX200 System.
11. Food testing
12. Perform routine evaluation of genetically modified organisms (GMO) using validated ddPCR methods.

Service Features

1. Flexible digital PCR chemistry-optimized for TaqMan hydrolysis probe and EvaGreen applications
2. Reduced bias from amplification efficiency and PCR inhibitors
3. Flexible assay setup-scalable for high sensitivity or high throughput
4. Convenient assay design-standard curves are not required
5. Simple workflow with 96-sample throughput
6. The QX200 Droplet Digital PCR system comprises:
7. QX200^™ droplet generator-partitions each ddPCR reaction mix into 20,000 nanoliter-sized droplets
8. QX200^™ droplet reader-streams emulsified droplets in single file past a two-color optical detection system in a serial manner
9. QuantaSoft^™ software-performs quantitation of positive/negative droplets and allows visualization in a variety of ways and determine concentrations in copies/µL
10. Associated components and consumables included: DG8^™ cartridges, DG8^™ gaskets, and cartridge holder

CD Genomics, as a specialist sequencing company, has a large range of sequencers in our NGS Core, including Illumina MiSeq, NovaSeq, PacBio, and more. If you require this service, please contact us directly.