Clone Expansion Services

Introduction

Thawing from 96-well plates of colonies chosen based on Southern blot results
Expansion to 24-well and then 6-well plates
Freezing 6-9 vials of cells per clone and storing in liquid nitrogen
Extraction of about 50 micrograms of genomic DNA for further genotyping

Summary

Gene targeting is the process of disrupting or mutating a specific genetic locus in embryonic stem (ES) cells, usually with the intention of making knock-out or knock-in mice. Other methods of mutating the mouse genome include chemical mutagenesis, insertion of viral vectors or transposons, and random integration of gene-trap or promoter-trap vectors. Use of Cas9 nuclease is probably the most popular method to mediate genome engineering directly in mouse embryos, without the need to use ES cells. Note, CRISPR engineering can also be used in ES cells to engineer changes in the genome, including insertion or substitution of relatively large regions of DNA.

CD Genomics has already updated its technology platforms to mainstream NGS, long-read sequencing, and microarray instruments. We continue to strive to offer the same reliable services to pharmaceutical and biotechnology companies, as well as academia and government agencies, for all your sequencing or array needs. If you are interested, please contact us directly for assistance.