Genomics Service—QuantStudio 5 qPCR System

Introduction

QuantStudio 5 has a HRM curve capabilities; a relatively new, post-PCR analysis method used to identify variations in nucleic acid sequences. The method is based on detecting small differences in PCR melting (dissociation) curves. It is enabled by improved dsDNA-binding dyes used in conjunction with real-time PCR instrumentation that has precise temperature ramp control and advanced data capture capabilities. Data are analyzed and manipulated using software designed specifically for HRM analysis. For HRM application mutation scanning and methylation analysis information please see the following links.

Summary

The Applied Biosystems QuantStudio 5 Real-Time PCR System with 384-well block is a real-time thermocycler that allows quantitative and qualitative detection of nucleic acids based on fluorescent-based technology. The optimized Design and Analysis software is ideal for both first-time and experienced users. The instrument includes wi-fi connectivity and when connected to the Thermo Fisher Cloud, the QuantStudio 5 system provides access to your data wherever and whenever you want. The 384-well format has 5 excitation filters (450-650 nm) and 5 emission filters (500-700 nm).

CD Genomics offers you QuantStudio 5 qPCR System based genomic services. If you are interested, please feel free to contact us for more information.