Single Cell Expression Profiling & Genomics Services

Introduction

Single Cell Gene Expression (3′ GEX V3.1)
Fixed Single Cell Gene Expression (human and mouse samples)
Single Cell Immune Profiling (5′ GEX V1.1 and V2 + V(D)J)
Single Cell ATAC-seq
HT Gene Expression and Immune Profiling
Visium Library Preparation (slide preparation not included)
Single Cell Multiome (combined ATAC + GEX)

Service Features

Fast workflow from cell suspension to 3′-cDNA or 5′-cDNA library.
Captures 100-20,000+ cells per sample and up to 320,000 cells (or ~ 1 million cells with multiplexing) per run in ~20 minutes.
Recovers up to ~65% of cells (typically 50%).
Low doublet rate (~0.9% per 1,000 cells).
Compatible with Illumina and Element Biosciences Aviti sequencers.
The 10X GEX Single-Cell libraries are most economically sequenced on the Illumina NovaSeq 6000 with paired-end reads. (The assay requires at least a 28 cycle forward read, a 10 bp dual index reads, and a 90 cycle reverse read).
For most applications, an average of 40,000 reads per cell should be sequenced (for cell types with complex transcriptomes). For isolated nuclei 25,000 reads each are recommended,

CD Genomics has already updated its technology platforms to mainstream NGS, long-read sequencing, and microarray instruments. We continue to strive to offer the same reliable services to pharmaceutical and biotechnology companies, as well as academia and government agencies, for all your sequencing or array needs. If you are interested, please contact us directly for assistance.