Reconstitution of Live Mice from Frozen Sperm Services

Introduction

Performing IVF with oocyte donors of the appropriate strain
Generating pseudopregnant foster moms
Transferring fertilized embryos to foster moms
Monitoring pregnancies
Weaning pups

Summary

The cryopreservation of mouse strains serves two main functions:
When mouse strains are not immediately needed for experimental purposes, but may be needed at a later date and cannot be readily obtained from vendors, it may be more economical to store the strain as frozen embryos or sperm, rather than maintaining a live colony.
Mouse strains that are difficult to replace should be cryopreserved to allow reconstitution in the event of a pathogen outbreak, natural disaster, or other occurrence that may result in the loss of all existing breeding stock.

Turnaround Times

Embryo cryopreservation usually requires about a week, once the embryo donors and stud males are designated by the client, and a freeze date has been scheduled. Sperm cryo usually requires one day. In vitro fertilization requires a minimum of 2 days.

CD Genomics has already updated its technology platforms to mainstream NGS, long-read sequencing, and microarray instruments. We continue to strive to offer the same reliable services to pharmaceutical and biotechnology companies, as well as academia and government agencies, for all your sequencing or array needs. If you are interested, please contact us directly for assistance.