Mating TTML Animals Services

Introduction

Mating Schemes for 129-Derived Chimeras
Mating Schemes for B6-Derived Chimeras

Summary

Separate (singly house) males at seven weeks of age to optimize sperm counts. Introduce two females (over six weeks old) to each singly housed male the following week. Check for the presence of a copulation plug every day for a week. Remove the first two females after seven days and add two fresh females to the cage. If plugs were observed, expect litters 19 days from day of plug. Note that males, particularly young males, will sometimes mate with females that are not in estrus so the females may not be pregnant or may drop a litter several days later than expected, if the male mates with her again when she is in estrus (the female estrous cycle occurs every four to five days). Plugs may be observed even if the male is sterile; in which case, no litters will ever be born

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