NGS Service—Illumina NextSeq DNA Sequencer

The main reason for using next-generation sequencing is the effective reduction in turnaround time with NGS compared to traditional methods. At the same time, next-generation sequencing makes it possible to analyze the transcriptome and genome of a species in a detailed and holistic way. CD Genomics offers a growing range of services, from library construction to sequencing to complete experimental design and support. Our Illumina NextSeq DNA Sequencer-based next-generation sequencing technology can help you advance genome engineering innovation.

Name of the Instrument/Technicial Service

Illumina NextSeq DNA Sequencer

Introduction

Specifications
1. The instrument utilizes a four lane flow cell which are combined as a single sequencing lane.
2. Sequencing times are very rapid with run times of 11 hours (75 bp single read) to 26 hours (300 bp paired-end read).
3. The NextSeq has a variety of different sequencing kits available including:
1) 75 bp single read high output (400 million+ reads)
2) 75 bp paired-end read high output
3) 150 bo paired-end read high output
4) 75 bp paired-end mid output (130 million+ reads)
5) 150 bp paired-end read mid output
4. The single lane flow cell is capable of generating 400 million+ sequence reads per lane passing filter. We routinely exceed 500 million reads pass filter per run.
5. Paired-end sequencing permits fragments to be sequenced from both ends, approximately doubling the output of sequence data and aiding in assembly of genomes.

MANUFACTURER: Illumina

Summary

The NextSeq is one of the newest sequencers from Illumina which is designed for greater read depth and fast sample processing. The NextSeq is one of the next generation of sequencers that will replace the HiSeq series in the coming years. The Genomics Core operates two of these instruments. It combines the rapid sequencing times of the MiSeq with high sequence output per run. The NextSeq 500 runs one sample at a time using a one lane flowcell. However, the flowcell lane is capable of yielding 400 million+ reads per run. This means higher multiplex levels and reduced cost per base. The sequencer can provide 1×75bp, 1×150bp, 2×75bp and 2×150bp outputs. There is also mid-output sequencing kits that provide up at a maximum of about 130 million reads per run. One sample per run and very rapid cycle times means no wait time to fill eight-lane flowcells as on the HiSeq and shorter sample processing times. The NextSeq500 is ideal for small or mid-sized projects with multiplexed samples. The most common research applications for this instrument are similar to those of the HiSeq series.

Applications

• Whole Genome sequencing/re-sequencing
• Genome-wide detection of SNPs and mutations
• DNA methylation profiling
• RNA expression profiling
• Small RNA profiling and discovery
• DNA protein interactions (ChIP sequencing)

CD Genomics, as a specialist sequencing company, has a large range of sequencers in our NGS Core, including Illumina MiSeq, NovaSeq, PacBio, and more. If you require this service, please contact us directly.