Advanced FISH Services

Introduction

We offer three main services, which can be delivered alone or in combination:
Design of DNA/RNA smFISH probes against any organism of interest for which a reference genome/transcriptome is available (service area 1)
Enzymatic production of smFISH probes starting from oligopools, either already available at our unit or custom-designed and purchased for the user (service area 2)
DNA/RNA smFISH on fixed cells, frozen and FFPE tissue sections (service area 3)

Summary

For smFISH experiments, we rely on our custom-built epifluorescence microscope (Nikon Ti-E) equipped with an ultra-sensitive camera (Andor) and add-on tools specifically tailored for smFISH.

Applications

RNA smFISH can be used to confirm differences in gene expression between cells and/or tissue regions or to study the spatial distribution of cells expressing a particular gene/set of genes of interest in a tissue (max 5 genes per sample) in a small subset of samples (less than 10)

Service Features

We prioritize our services according to the following criteria (in decreasing order of importance):
1) Service required for the revision of a manuscript by a user in academia > Service not needed for an imminent publication
2) Probe design and production > Full FISH service
3) Academic users > Private company users

CD Genomics has already updated its technology platforms to mainstream NGS, long-read sequencing, and microarray instruments. We continue to strive to offer the same reliable services to pharmaceutical and biotechnology companies, as well as academia and government agencies, for all your sequencing or array needs. If you are interested, please contact us directly for assistance.