NGS Service—Applied Biosystems Quantstudio 5

Introduction

qPCR is a method of measuring how much DNA in a sample contains a region (or regions) of interest. Like standard PCR, precisely-designed primers target specific regions that are (or may be) within the sample, and replicate that region over the course of 25-50 thermal cycles. Unlike standard PCR, the DNA is dyed with a fluorophore and the intensity of the fluorescence is measured at the end of each replication cycle. The cycle at which the fluorescence reaches measurable levels above background noise is referred to as the threshold cycle (CT). Control samples with known input quantities of DNA are run simultaneously, and their CT values are used to generate a standard curve so that the quantity of the experimental sample can be accurately calculated.

Summary

The QuantStudio 5 Real-Time PCR is a high-performance instrument equipped with a 384-well block. The system offers measurement of a broad range of genomic applications allowing use of probe-based assays and intercalating dyes.

CD Genomics, as a specialist sequencing company, has a large range of sequencers in our NGS Core, including Illumina MiSeq, NovaSeq, PacBio, and more. If you require this service, please contact us directly.