Mouse In Vitro Fertilization Services

Introduction

Mouse in vitro fertilization is usually used to recover strains that have been cryopreserved or to aid strains that have stopped breeding naturally. It is also used to rederive lines that have unknown health status or are known to have pathogens. Oocytes are collected from 5 superovulated females and incubated with sperm (either fresh or frozen) and cultured to the 2-cell stage after which the fertilized embryos are transferred to pseudopregnant CD1 females. The researcher will received all pups after weaning.

Instrumentation

Hamilton Thorne Computer-Aided Sperm Analysis System
Sutter Xenoworks Micromanipulator
Sutter Xenoworks Digital Microinjector
Nikon TE2000 Inverted Microscope
Leica M165 Dissecting Microscope
MINC Benchtop Incubator
Sutter Micropipette Puller
Fine Science Tools Bead Sterilizer
Baker EdgeGUARD Clean Bench
TMC Air Isolation Table

CD Genomics has already updated its technology platforms to mainstream NGS, long-read sequencing, and microarray instruments. We continue to strive to offer the same reliable services to pharmaceutical and biotechnology companies, as well as academia and government agencies, for all your sequencing or array needs. If you are interested, please contact us directly for assistance.