Pin Transfer Service—K2 Compound Transfer Robot (SGM 611)

Introduction

Design: Richard Siu
Construction: Richard Siu & Alasdair Morton
Integration Software: Custom C# .Net Application developed by Richard Siu
Robotic:
Peak Robotics KX2-750 robot arm
Peak Robotics CS6 carousel
Epson T6-602s SCARA robot
Table: Custom Designed by SteelSentry
Sonicator: Sonicor SC-50
Pin Arrays:
33 nL
100 nL
300 nL
Cycle Time: Approximately 1 minute per transfer, including wash and dry
Plate Types: Accepted 384-well
Total Plate: Capacity 150
The amount of compound transferred is dependent on several parameters
1. Pin shape and diameter
2. Volume of stock solution
3. Rate of pin withdrawal from the stock solution
4. Viscosity of liquid in the assay plate
5. Dwell time in the assay plate
To prevent compound carryover, each pin array is washed after every library transfer.
Pin Washing Steps:
1. PBS
2. 100% methanol flow-through wash
3. 20% methanol sonication step
4. Repeat twice
5. Concludes with a 10-second high-pressure air dry

Summary

We utilizes two different robotic platforms to conduct pin transfer of nanoliter volumes from compound storage plates into assay plates using stainless steel pin arrays.
1. 96-and 384-well pin arrays
2. Transfer compounds into 96-, 384-and 1536-well microplates
3. 384-well assay plate volume 30 µL-calibrated to transfer 33 nL, 100 nL or 300 nL
4. 384-well assay plate volume 10 µL-calibrated to transfer 33 nL or 100 nL
5. The standard transfer volume for chemical screens in 384-well is 100 nL.
6. 96-well assay plate volume 150 µL-calibrated to transfer 200 nL, 250 nL or 300 nL

CD Genomics develops well-thought-out Pin transfer solutions based on customer needs. If this is of interest to you, please contact us directly for information.