Molecular Services

CD Genomics is the pioneer in genomics research. Our experts and technicians are knowledgeable and have years of experience providing Molecular Services.

Name of the Instrument/Technicial Service

Introduction

Our team offers CRISPR gene knock-out services for cell lines and CRISPR KI/KO/CKO in mouse lines.

Our Services

Our services include but are not limited to:
Genotyping (including genomic DNA preparation):
PCR screening, genotyping, genotyping protocol
PCR genotyping program trouble shooting and redesigning
Long Range-PCR (6-7kb)
Designing, Construction and Preparation of Transgene Vector:
Plasmid
BAC
Designing, Construction and Preparation of Gene Targeting Vector:
Conventional knock-out
Conditional knock-out
Conditional restoration
Knock-in (e.g., point mutation, humanization, reporter, ROSA26)
Conditional expression of transgene from ROSA26 locus

Analysis Methods

Guide RNA design, construct and plasmid preparation
Transfection with guide RNA, Cas9
Use of selection markers such as puromycin and GFP to enrich for transfected cells
CRISPR activity analysis: SURVEYOR mutation detection assay
Single clone generation: sorting cells plus expansion
Gene knock out confirmation by Western blot analysis
Gene knock out confirmation by DNA sequencing
Final deliverable: gene knock out cell line and a complete report

CD Genomics offers various solutions, from genome-wide association studies on tens of thousands of samples to single SNP interrogations of several hundred individuals. Our experts and technicians are knowledgeable and have years of experience providing Molecular Services.

Online Inquiry

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