Genomics Service—CRISPR Platform

Introduction

1. Synthetic CRISPR Platform
The synthetic CRISPR platform enables gene knockout in an arrayed format using liquid handling automation in 96- or 384-well format. This platform uses synthetic purified sgRNAs that are transiently delivered into Cas9-stable cells using lipid transfection reagents or nucleofected with purified Cas9 protein. The phenotype is assessed typically within 72-96 hours. This platform enables cell phenotyping approaches using high content imaging, fluorescence/luminescence plate reader end points or FACS profiling.
1) Resources available:
• Human whole genome arrayed sgRNA library (Horizon Discovery)
a. Screen as a whole genome (61 plates) or cherry pick custom sub-sets
• Arrayed synthetic single crRNA kinome
• Cas9 virus or protein
• Nucleofection reagents
• Purchasing sgRNAs at discounted rates
2. Viral CRISPR Platform
We house both pooled and arrayed viral CRISPR libraries. Pooled CRISPR library are analysed using next generation sequencing. We take you through the screening process step-by-step for both whole genome pooled library or custom pooled sublibrary screens, from planning to execution through to analysis. The TransEDIT dual guide viral library in arrayed format can be used for single target validation, or boutique pooled library screening.
1) Resources available:
• CRISPR KO Brunello (human) and Brie (mouse) pooled libraries
• CRISPRa Calabrese (human) and Caprano (mouse) pooled libraries
• CRISPRi Docetto (human) and Dolomiti (mouse) pooled libraries
• Custom pooled CRISPR sublibrary generation and reagents
• Library amplification protocols and reagents
• In-house NGS and analysis pipelines
• Cas9 and dCas9 virus
• TransEDIT dual guide whole genome human arrayed viral library

Summary

CRISPR screens enable researchers to determine resistance in their cellular model, facilitate biomarker discovery in patient samples or determine genes responsible for synthetic lethality. We supports both synthetic and pooled CRISPR screening strategies.

CD Genomics offers you CRISPR Platform-based genomic services. If you are interested, please feel free to contact us for more information.