ESC-mediated Gene Targeting Services

CD Genomics offers various solutions, from genome-wide association studies on tens of thousands of samples to single SNP interrogations of several hundred individuals. Our experts and technicians are knowledgeable and have years of experience providing ESC-mediated Gene Targeting Services.

Name of the Instrument/Technicial Service

ESC-mediated Gene Targeting Services

Introduction

Gene targeting in mice involves several important steps:

Engineering a gene targeting construct
Transfection of embryonic stem (ES) cells
Assessing ES cells for homologous recombination
Generating chimeric mice by blastocyst injection
Assessing germline transmission
Breeding chimeric mice to homozygosity

Our Services

Based upon our observations with successful targeting constructs, we urge you to design your construct to incorporate at least the following characteristics:
use DNA from mouse strain isogenic to the ES cell line to be used to optimize recombination efficiency. We typically recommend 129/Sv or C57B1/6, but other ES cell strain backgrounds are available.
provide at least 5 kb of total flanking homology (minimum 1 kb for either targeting arm; recommended minimum 2kb)
provide a +/– selection system (e.g. neo/Tk, neo/DT)
use a ubiquitous promoter, such as PGK or PMC, to drive the neo gene (conferring resistance to G418)

Summary

Our team creates genetically engineered (“knock-out”) mice via homologous recombination in embryonic stem (ES) cells. Our services can be ordered together or individually depending on the investigator's needs.

CD Genomics has already updated its technology platforms to mainstream NGS, long-read sequencing, and microarray instruments. We continue to strive to offer the same reliable services to pharmaceutical and biotechnology companies, as well as academia and government agencies, for all your sequencing or array needs. If you are interested, please contact us directly for assistance.

Online Inquiry

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