Transfection, Selection and Assessment of Embryonic Stem (ES) Cells Services

Introduction

Your construct will be transfected into ES cells by electroporation. Following successful electroporation, selection and clonal expansion, we will provide you with approximately 200 clones (unless a different number is required) on 24-well plates from which you will extract DNA and screen for homologous recombination. We freeze parallel 24-well plates at –70C, so screening must be performed promptly. Individual clones demonstrating homologous recombination events will be thawed at your request and you will receive a second dish of cells to confirm the initial screening assessment.

CD Genomics has already updated its technology platforms to mainstream NGS, long-read sequencing, and microarray instruments. We continue to strive to offer the same reliable services to pharmaceutical and biotechnology companies, as well as academia and government agencies, for all your sequencing or array needs. If you are interested, please contact us directly for assistance.