Targeting Vector Design Services

Introduction

1. Introducing Deletional Mutations (Knock-Out Models)
2. Introducing Point Mutations (Knock-In Models)
3. Positive Selection Markers
4. Negative Selection Markers

Summary

The basic targeting vector contains 5' and 3' arms of sequence homologous to the gene being targeted and positive/negative selection markers used to disrupt gene function and/or to identify ES cell clones that integrated targeting vector DNA following electroporation. Elements for the deletion of incorporated sequences at later stages of development or in specific tissues can be included. Inclusion of a point mutation that creates a different restriction pattern should be considered if necessary to distinguish integrated DNA sequence from endogenous sequences during ES cell clone screening.

CD Genomics has already updated its technology platforms to mainstream NGS, long-read sequencing, and microarray instruments. We continue to strive to offer the same reliable services to pharmaceutical and biotechnology companies, as well as academia and government agencies, for all your sequencing or array needs. If you are interested, please contact us directly for assistance.