Pronuclear Microinjection of DNAs Services

CD Genomics offers various solutions, from genome-wide association studies on tens of thousands of samples to single SNP interrogations of several hundred individuals. Our experts and technicians are knowledgeable and have years of experience providing Pronuclear Microinjection of DNAs Services.

Name of the Instrument/Technicial Service

Pronuclear Microinjection of DNAs Services

Introduction

This service provides an efficient means of introduction foreign DNA, RNA, and ribonucleoproteins into the mouse genome. DNA or RNA is microinjected into the pronucleus or cytoplasm of 0.5 day fertilized mouse embryos. Surviving embryos are transferred to the oviduct of a pseudopregnant recipient and the animals are housed under the protocol number of the investigator but husbandry is provided by the VGER and DAC. The gestation period of the mouse is 19 to 21 days. Pups are born and will be weaned, tailed and numbered by core personnel at 3-4 weeks of age. The investigator will need to perform DNA isolation and analyze the DNA by PCR or Southern blotting to identify transgenic founders.

Protocol

Service requests are reviewed and approved by the Co-Directors of the facility.
PI submits frozen DNA. DNA is quantitated, quality controlled and diluted by VGER staff.
Injections are scheduled, mice are ordered.
Injections are performed, surviving embryos are transferred to pseudopregnant females.
Pups born 19 to 20 days post-injection.
Pups weaned, tailed and ear punched by VGER staff at 3 weeks of age.
Dams are serology tested at weaning.
PI identifies transgenic founders from tail DNA using PCR or Southern blotting.

CD Genomics has already updated its technology platforms to mainstream NGS, long-read sequencing, and microarray instruments. We continue to strive to offer the same reliable services to pharmaceutical and biotechnology companies, as well as academia and government agencies, for all your sequencing or array needs.

Online Inquiry

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