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NGS Service—PacBio Sequel IIe

The main reason for using next-generation sequencing is the effective reduction in turnaround time with NGS compared to traditional methods. At the same time, next-generation sequencing makes it possible to analyze the transcriptome and genome of a species in a detailed and holistic way. CD Genomics offers a growing range of services, from library construction to sequencing to complete experimental design and support. Our PacBio Sequel IIe-based next-generation sequencing technology can help you advance genome engineering innovation.

Name of the Instrument/Technicial Service

PacBio Sequel IIe

Introduction

TECHNICAL DETAILS
1.Each Sequel IIe SMRT cell has ~ eight times the sequencing capacity of a Sequel SMRT cell
2.8M wells per SMRT cell resulting typically in more than 5M reads
3.As much as 150 Gb per flow cell
4.Read lengths are determined by your sample and experimental needs
5.High Quality per base (< 1% error rate) in HiFi reads
6.Sequel IIe sequences (native) DNA — meaning no amplification bias

MANUFACTURER: Pacific Biosciences

Summary

Specifications
1. Each SMRTcell can produce up to 4 million reads
2. High Fidelity (HiFi) reads with > 99% accuracy from templates of 1-20 kb in length can be generated from circular consensus
3. Multiplexing available for Iso-seq, amplicon, and bacterial genome sequencing

Applications

Used for a variety of applications:
1. De novo whole genome sequencing
2. Iso-seq
3. Amplicon Sequencing
4. Methylation Detection

CD Genomics, as a specialist sequencing company, has a large range of sequencers in our NGS Core, including Illumina MiSeq, NovaSeq, PacBio, and more. If you require this service, please contact us directly.

For research use only, not intended for any clinical use.

Online Inquiry

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