NGS Service—Oxford Nanopore GridION and PromethION

Summary

The Oxford Nanopore platform is the newest and most rapidly evolving platform in our portfolio of sequencing technologies. Monitoring current fluctuations as native DNA or RNA molecules pass through nanopores, this approach has key advantages of being able to read µLtra-long fragments (some > 1 Mb) and detecting modified bases without special library construction. Base calling is done in real time making sequence data available right from the start of the run. Raw read accuracy is currently about 98% with new advances expected to push that over 99%. The primary uses of this technology to date at NISC have taken advantage of the µLtra-long reads to detect large structural variants and create de novo genome assemblies. Using this approach, a typical PromethION flowcells yields 80 Gbase of quality reads with N50 approximately 72 kb. Preliminary experiments have also been undertaken to explore direct RNA and full-length cDNA sequencing.

CD Genomics, as a specialist sequencing company, has a large range of sequencers in our NGS Core, including Illumina MiSeq, NovaSeq, PacBio, and more. If you require this service, please contact us directly.