NGS Service—Illumina NextSeq® 550

The main reason for using next-generation sequencing is the effective reduction in turnaround time with NGS compared to traditional methods. At the same time, next-generation sequencing makes it possible to analyze the transcriptome and genome of a species in a detailed and holistic way. CD Genomics offers a growing range of services, from library construction to sequencing to complete experimental design and support. Our Illumina NextSeq^® 550-based next-generation sequencing technology can help you advance genome engineering innovation.

Name of the Instrument/Technicial Service

Illumina NextSeq^® 550

Introduction

Data Generation
1. Throughput per run
1) Up to 400 million reads per run (eg, clusters passing filter) using High Output flow cell
2) Up to 130 million reads per run (eg, clusters passing filter) using Mid Output flow cell
2. Sequencing flexibility
1) 100–120 Gb data per 2 × 150 bp run using High Output flow cell and reagents
2) 33–40 Gb data per 2 × 150 bp run using Mid Output flow cell and reagents
3) Set-up options include single-read or paired-end runs
3. Array Scanning
1) Flow cell options (eg, Mid or High Output flow cells) can be used to select data output levels
2) Read length is fully adjustable up to 300 base pairs
3) Single array imaged per scanning session
4) Up to 12 samples imaged per run with current array support
Instrumentation
1. Illumination
1) 12 light-emitting diodes at 520 nm, 650 nm
2. Instrument Control Computer
1) Dual Intel Xeon E5-2448L 1.8 GHz CPU with 96 GB of RAM included for instrument control, processing images, and base calling
2) Conducts real-time analysis processing that automatically produces image intensities and quality-scored base calls directly on the instrument computer
3. Sequencing run time
1) ~ 12 hours for a 1 × 75 bp single-read sequencing run
2) ~ 18 hours for a 2 × 75 bp paired-read sequencing run
3) ~ 30 hours for a 2 × 150 bp paired-read sequencing run

MANUFACTURER: Illumina

Summary

The Illumina NextSeq^® 550 is the largest desktop next-generation sequencing (NGS) system from Illumina capable of sequencing a 30× human genome in a single run. Two different flow cell options and multiple reagent configurations are providing a maximum application flexibility (130 to 400M clusters; 40 to 120 Gb).
Accordingly, the system is suitable for a high number of different applications, e.g. whole exome- or genome sequencing, RNAseq, targeted resequencing or gene expression profiling and single-cell sequencing. The sequencing technology is based surface-based bridge amplification for clonal amplification and sequencing of each generated cluster using the Illumina "sequencing by synthesis" (SBS) method with reversible terminator chemistry.

Service Features

1. Scalability (20–120 Gb) in a single run supporting a broad range of applications and study sizes
2. Sequencing runs, including on-board cluster generation, complete in 12–30 hours
3. Proven SBS chemistry with single-base extension enables accurate sequencing of homopolymers (sometimes a problem on the IonTorrent system)
4. Fully automated paired-end sequencing
5. Automated BeadChip array scanning and image file generation
6. Approximately 7000 peer-reviewed publications have been published using Illumina SBS sequence data, and more than 24,000 peer-reviewed publications have been published using Illumina array technology
7. Number of reads per run
8. preadjustment of library diversity is required for optimum sequence quality

CD Genomics, as a specialist sequencing company, has a large range of sequencers in our NGS Core, including Illumina MiSeq, NovaSeq, PacBio, and more. If you require this service, please contact us directly.