NGS Service—Illumina MiSeq DNA Sequencer

The main reason for using next-generation sequencing is the effective reduction in turnaround time with NGS compared to traditional methods. At the same time, next-generation sequencing makes it possible to analyze the transcriptome and genome of a species in a detailed and holistic way. CD Genomics offers a growing range of services, from library construction to sequencing to complete experimental design and support. Our Illumina MiSeq DNA Sequencer-based next-generation sequencing technology can help you advance genome engineering innovation.

Name of the Instrument/Technicial Service

Illumina MiSeq DNA Sequencer

Introduction

1. This instrument if capable of processing a flow cell containing 1 lane but is rapid with run times of 8 hours (50 bp single read) to about 48 hours (300 bp paired-end read).
2. The single lane flow cell is capable of generating up to 25 million sequence reads per lane (V3 kit).
3. There is a range of sequencing kit available for the MiSeq including:
1) 50 bp single read (up to 15 million reads)
2) 150 bp single end/75bp paired end (up to 15 million reads)
3) 300 bp single read/150 bp paired end (up to 25 million reads)
4) 250 bp paired end read (up to 15 million reads)
5) 300 bp paired end read (up to 25 million reads)
6) Micro kit 300 bp single read/150 bp paired end (up to 4 million reads)
7) Nano kit 250 bp paired end (up to 1 million reads)
8) Nano kit 300 single read/150 paired-end (up to 1 million reads)
4. Micro and Nano kits are ideal for small-scale or preliminary experiments.
5. Paired-end sequencing permits fragments to be sequenced from both ends, approximately doubling the output of sequence data and aiding in assembly of small genomes such as those of bacteria and fungi.

MANUFACTURER: Illumina

Applications

1. This instrument is used by core personnel for sequencing DNA libraries.
2. The MiSeq is a lower throughput sequencer that generate fewer reads (maximum of 25 million reads V3 kits) than the NextSeq but is excellent for applications such as metagenomics studies involved the sequencing of amplicons generated by PCR such as 16s ribosomal DNA.
3. Read lengths range between 50bp single-end to 300 bp paired-end (600 bp). The instrument provides a variety of useful outputs, including small genome re-sequencing, small genome de novo sequencing and amplicon sequencing for metagenomics studies.
4. A variety of kits are available commercially to users to produce libraries for sequencing including low input kits.
5. Customers can produce their own libraries for sequencing. A common protocol for 16S metagenomics is that offered by Illumina (16s-metagenomic-library-prep-guide-15044223-b) which takes advantage of Nextera dual index barcodes.

CD Genomics, as a specialist sequencing company, has a large range of sequencers in our NGS Core, including Illumina MiSeq, NovaSeq, PacBio, and more. If you require this service, please contact us directly.