NGS Service—DNA Sequencer

Introduction

Specifications
1. This is a capillary sequencer capable of processing 96 template samples simultaneously; up to ~4000 samples/week.
2. Standard sequencing PCR reaction conditions are:
3. 96 degree C denaturation 30 seconds
4. 50 degree C extension for 15 seconds
5. 60 degree C extension for 4 minutes 30 seconds
6. A total of 35 cycles are used

Applications

1. This instrument is used by core personnel to sequence DNA templates
2. Sequencing of cloned inserts or PCR products is done by DNA polymerase-mediated linear amplification of templates in the presence of mixtures of dNTPs and fluorescently-labeled dideoxynucleotide triphosphates (Sanger method), followed by fractionation of the extension products by capillary electrophoresis using the 3730×XL.
3. Although Sanger sequencing is well established and lower throughput that modern NGS sequencers, it is still the gold standard in terms of accuracy and is still used worldwide for the low cost sequencing of plasmid and PCR templates.

CD Genomics, as a specialist sequencing company, has a large range of sequencers in our NGS Core, including Illumina MiSeq, NovaSeq, PacBio, and more. If you require this service, please contact us directly.