NGS Service—Covaris M220 Ultrasonicator

The main reason for using next-generation sequencing is the effective reduction in turnaround time with NGS compared to traditional methods. At the same time, next-generation sequencing makes it possible to analyze the transcriptome and genome of a species in a detailed and holistic way. CD Genomics offers a growing range of services, from library construction to sequencing to complete experimental design and support. Our Covaris M220 Ultrasonicator-based next-generation sequencing technology can help you advance genome engineering innovation.

Name of the Instrument/Technicial Service

Covaris M220 Ultrasonicator

Introduction

This instrument uses focused sound waves to fragment DNA in a controlled and random manner: the time exposed to the sonication determines the final fragment length, and the method of shearing ensures that no bias is present in the resulting pool of fragments-as was a concern with early enzymatic shearing methods.
The M220 is a single-sample machine, primarily designed for shearing DNA in preparation for next-generation sequencing library builds. However, the core also has an adapter for the instrument which will allow the adaptive focused acoustic (AFA) process to be applied to cells or FFPE tissue for extraction of DNA or RNA, and has assisted several researchers with that type of protocol as well.

Summary

The Covaris M220 Ultrasonicator provides mechanical shearing of DNA to generate tight, highly reproducible fragment distributions. Optimized shearing protocols are available for fragment sizes from 150 to 5,000 bp.

Technical Capabilities

DNA shearing

CD Genomics, as a specialist sequencing company, has a large range of sequencers in our NGS Core, including Illumina MiSeq, NovaSeq, PacBio, and more. If you require this service, please contact us directly.

Online Inquiry

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