MAPseq/BARseq Services

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Genomics-Related Laboratory Services

CD Genomics is the pioneer in genomics research. Our experts and technicians are knowledgeable and have years of experience providing MAPseq/BARseq Services.

Name of the Instrument/Technicial Service

MAPseq/BARseq Services

Introduction

BARseq (barcoded anatomy resolved by sequencing) is a high-throughput, multiplexed method based on RNA barcoding that helps bridge anatomical and transcriptomic approaches at cellular resolution with the potential to discover organizing principles of neural circuits.

Our Services

Quantify the projections of thousands—or even millions—of individual neurons in parallel within a single brain.
Produce multiple projection patterns from one to several injection sites, at single-neuron resolution.
Determine the brain-wide projections from a given area.
Compare projection patterns originating from different areas of the brain.
Determine whether individual neurons from a given area project to a specific area of interest.
Perform single-neuron tracing in less common animal model systems, including rodents and potentially non-human primates.

Protocol

BARseq can be used to:
Sequence cellular barcodes in intact brain slices, preserve anatomical information and examine connectivity at greater resolution.
Map multiple measures in the same neurons: the connections, neuron activity via calcium imaging, and gene-expression data.
Expand the brain map by accurately pinpointing the location of a neuron.
Be potentially used for barcode-assisted lineage tracing, and map long-range axonal projections.
Be used for genetic labeling.

CD Genomics offers various solutions, from genome-wide association studies on tens of thousands of samples to single SNP interrogations of several hundred individuals. Our experts and technicians are knowledgeable and have years of experience providing MAPseq/BARseq Services.

Online Inquiry

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