High Molecular Weight DNA Isolation (HMW-DNA) Services

Introduction

Since long-read sequencing protocols (PacBio, Nanopore) analyze single DNA molecules, success depends on high-quality DNA samples. 10X Genomics linked-read sequencing has similarly high sample requirements with regards to purity and integrity, but requires far lower amounts. Thus we are now offering high-molecular-DNA (HMW-DNA) isolations as a service. The applied DNA isolation protocols will vary with the sample types. Our protocols aim to isolate HMW-DNA samples with mean fragment lengths of 50 kb or higher, if the sample allows. Longer fragments can be expected for examples for cell culture or fresh blood samples. However, we can’t promise any specific performance metrics. For larger projects we will test isolations first. The HMW-DNA isolation service includes DNA sample QC by pulsed field electrophoresis (PFGE), spectrometry and fluorometry.

CD Genomics has already updated its technology platforms to mainstream NGS, long-read sequencing, and microarray instruments. We continue to strive to offer the same reliable services to pharmaceutical and biotechnology companies, as well as academia and government agencies, for all your sequencing or array needs. If you are interested, please contact us directly for assistance.