Genomics Service—RNAi Platform

Introduction

1. siRNA, miRNA, long non-coding RNA Platform
The siRNA platform is based on Horizon Discovery's siGENOME SMARTpool reagents arrayed in either 96- or 384-well plate format. siRNAs targeting the protein coding genome, miRNAs (mimic or inhibitor) or long non-coding RNAs are transiently introduced into cells using lipid-based reagents and assayed between 72-144 hours post-transfection. This platform offers similar readout options as screening arrayed CRISPR.
1) Resources available:
• Protein coding siRNA (arrayed, whole genome or boutique screens)
• Human and mouse - siGENOME and siON-TARGET-plus libraries
• miRNA over-expression and inhibition (human)
• Long non-coding RNA siRNA (human)
2. shRNA Platform
We have a pooled shRNA screening platform to enable stable knockdown in primary, non-dividing and standard cell lines. shRNAs can be screened singularly for validation purposes or screened as genome-wide or custom pools.
1) Resources available:
• Pooled and arrayed human shRNA-mir30 whole genome library

CD Genomics offers you RNAi Platform-based genomic services. If you are interested, please feel free to contact us for more information.