Genome Engineering Services

Introduction

gRNA and/or donor design, assembly and validation in cultured cells for investigators who are making an animal model or wish to do cell line engineering themselves
Cancer cell line engineering-to introduce investigator-specified genetic modifications, including knockout and knockin, into the genome of a cancer cell line chosen by the investigator
Stem cell engineering--to introduce investigator-specified genetic modifications, including knockout and knockin, into the genome of an iPS or human ES cell line chosen by the investigator
NGS-based genotyping for cell lines and tissues from investigators who are making an animal model or wish to do cell line engineering themselves
Patient fibroblasts (from skin biopsy), renal epithelial cells (from urine sample), and PBMCs (from whole blood) isolation, expansion and banking
Reprogramming patient cells from above to iPSCs and characterization of the iPSCs, such as karyotyping, pluripotency staining and embryoid body formation and germ layer staining
Pluripotency staining of iPSCs and hESCs
Embryoid body formation of iPSCs and hESCs
Differentiation of iPSCs or hESCs to neuronal lineages, cardiomyocytes, hematopoietic stem cells, macrophages, etc.
Hands-on, on-demand training on iPSC culturing
NGS-based STR profiling for cell line authentication
Mycoplasma testing
CRIPSR library design and construction and screening and analysis
General molecular biology assistance

Instrumentation

Biomek FX
Appliedbiosystems Quant Studio3 Real-Time PCR system
Lonza 4D X-unit Nucleofector
Sony Cell Sorter SH800
Cell Metric Imager (Solentim)
Biotek Synergy HTX Multi-mode Reader
Biotek MultiFlo Washer and Dispenser
Tecan Fluent liquid handler
IncuCyte Imager S3

CD Genomics has already updated its technology platforms to mainstream NGS, long-read sequencing, and microarray instruments. We continue to strive to offer the same reliable services to pharmaceutical and biotechnology companies, as well as academia and government agencies, for all your sequencing or array needs. If you are interested, please contact us directly for assistance.