Gene Expression Profiling with 3′ Tag-Seq Services

Introduction

We are offering 3′ Tag-Seq as full-service packages (Batch-Tag-Seq, please see below) including library preps, sequencing and optional DGE data analysis. These packages are priced at easy-to-budget per-sample recharge rates.
For demanding sample types that require protocol modifications we are offering custom 3′ Tag-Seq sequencing – please inquire.

Service Features

Advantages of 3' Tag-Seq:
low noise gene expression profiling
less sensitive to RNA sample quality/integrity variations (compared to poly-A enrichment protocols)
>99% strand-specific; same direction as mRNA transcripts
requires significantly lower numbers of sequencing reads
single read sequencing is sufficient
simpler library prep protocol
costs about half or less compared to standard RNA-seq
costs lower than, or comparable to, microarray analysis
much higher dynamic range compared to microarrays
we routinely sequence 48 libraries per NextSeq 500 run with single-end 80bp reads or 96 samples per Aviti sequencer run; for some projects, it is possible to double these numbers
by default molecular barcodes will be incorporated: especially helpful for very low

CD Genomics has already updated its technology platforms to mainstream NGS, long-read sequencing, and microarray instruments. We continue to strive to offer the same reliable services to pharmaceutical and biotechnology companies, as well as academia and government agencies, for all your sequencing or array needs. If you are interested, please contact us directly for assistance.