DNA Shearing and Size Selection—Sage Scientific Blue Pippin

Introduction

Specifications
1. For DNA size selection cassettes are available from 0.75% to 3%. Gels can be stained or unstained and with or without internal size standards. We typically use unstained gels with internal standard for Illumina sample prep.
2. The Blue Pippin has both standard and pulse field electrophoresis capability permitting a large range of fragment size selection.
3. Cassettes can run up to 5 samples. Unused sample lanes can be used at a later time in some cases.
4. Gel cassettes available are:
1) 3% agarose 100-250 bp isolation
2) 2.5% agarose 100-600 bp
3) 1.5% agarose 250 bp - 1.5 Kb
4) 0.75% agarose 1 - 50 Kb
5. For protein size selection there are special 3% and 5% agarose gels run with 0.1% SDS.

Applications

1. This instrument can used by customers to precisely size select DNA fragments of desired length.
2. Computer controlled electrophoresis and sample gating permit fragments from about 90bp up to more than 50Kb to be isolated.
3. For DNA size selection samples are run on a cassette containing agarose gel.
4. The primary application of size selection of DNA is for preparing samples for Illumina and PacBio sequencing. DNA fragments can either be size selected prior to library prep or completed libraries can be size selected to remove primer dimers and other undesirable products.
5. Uses can also include miRNA isolation.

If you are interested, please contact us directly for assistance.