DNA Microinjection Services

Introduction

Purchase of egg donors.
Superovulation or hyperovulation of egg donors and mating with stud males.
Production of pseudopregnant foster mothers by mating outbred ICR females with vasectomized males and checking for vaginal plugs the morning of injection.
Harvesting of eggs from euthanized donors.
Dilution of the client’s transgene DNA with injection buffer to a concentration of 2 ng/ul and injection of this solution into one pronucleus of each fertilized egg.
Transfer of all eggs that survive the injection process into the oviducts of pseudopregnant foster mothers.
Monitoring of foster mothers before and after birth of pups.
Marking pups in each litter by distal toe-clipping, sexing, and (if required) tail-cutting at about ten days of age.
Transfer of toe or tail biopsies to the client for genotyping.
Weaning, identification, and transfer or shipment of transgenic pups to the client.

Summary

We offer 2 strains of egg donors on a routine basis. The service fee varies by strain because of differences in egg and pup yields among these strains.

Turnaround Times

Following receipt of a construct ready for microinjection along with the necessary paperwork, the minimum time required to produce transgenic founders that can be transferred to the client is about 8 weeks. This includes about 2 weeks to order and prepare the egg donors, 3 weeks gestation time, and 3 weeks from birth to weaning.

CD Genomics has already updated its technology platforms to mainstream NGS, long-read sequencing, and microarray instruments. We continue to strive to offer the same reliable services to pharmaceutical and biotechnology companies, as well as academia and government agencies, for all your sequencing or array needs. If you are interested, please contact us directly for assistance.