CRISPR-Cas9 Mouse Editing Services

CD Genomics offers various solutions, from genome-wide association studies on tens of thousands of samples to single SNP interrogations of several hundred individuals. Our experts and technicians are knowledgeable and have years of experience providing CRISPR-Cas9 Mouse Editing Services.

Name of the Instrument/Technicial Service

CRISPR-Cas9 Mouse Editing Services

Introduction

CRISPR-Cas9 technology has emerged as a rapid and precise method to generate genome edited mouse models. We offer full service mouse projects from genome editing strategy design through validation of the heterozygous N1 generation. We also offer injection services, CRISPR reagent sourcing, and design review for investigators wishing to design their own models using CRISPR-Cas9 technology.

Protocol

A genome editing strategy is devised and reviewed with the investigator. We have four classes of CRISPR-Cas9 genome editing projects based on the donor DNA format, length of the desired gene edit, and whether the edit is an insertion or replacement.
Guide RNAs are sourced in a crRNA + tracrRNA format and tested for activity in a test tube assay. Guides that pass the test tube activity assay are usually determined to be sufficiently active in vivo, but more complex projects that require mutliple guides may benefit from pre-screening in vivo.
CRISPR editing reagents and a donor DNA, if required, are delivered into the pronuclei of one-cell fertilized mouse zygotes. Our standard strain options is C57BL/6J. Other strains may also be used for genome editing. Inquire for more information.
Resulting pups are biopsied and screened by PCR and Sanger sequencing.
Predicted founders are transferred to the investigator for breeding and validation of the desired genome edit in the N1 generation.
Investigators are provided with a validated genotyping protocol and a genome editing report that includes a description of what the model is, how it was designed and produced, and additional critical information such as correct nomenclature for the new allele.

CD Genomics has already updated its technology platforms to mainstream NGS, long-read sequencing, and microarray instruments. We continue to strive to offer the same reliable services to pharmaceutical and biotechnology companies, as well as academia and government agencies, for all your sequencing or array needs. If you are interested, please contact us directly for assistance.

Online Inquiry

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